GENETICS AND GENETIC IMPROVEMENT OF DISEASE RESISTANCE AND QUALITY TRAITS IN WATERMELON, BROCCOLI, AND LEAFY GREEN BRASSICAS
Location: Vegetable Research
Title: Markers Linked to the ZYMV-FL Resistance Gene and Their Use in Marker-assisted Selection in Watermelon
Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2009
Publication Date: June 1, 2009
Citation: Harris-Shultz, K.R., Ling, K., Meyer, J.D., Levi, A., Wechter, W.P., Havey, M.J., Guner, N., Wehner, T. 2009. Markers Linked to the ZYMV-FL Resistance Gene and Their Use in Marker-assisted Selection in Watermelon. HortScience. 44:576.
Zucchini yellow mosaic virus (ZYMV) is a major pathogen that reduces yield in cucurbits. The best studied strains include ZYMV-FL and ZYMV-CH of which resistance is conferred by a single recessive gene. Bulk segregate analysis was used for watermelon populations derived from PI 595203, a line with ZYMV-FL and ZYMV-CH resistance, and NHM, a susceptible cultivar. A single marker, Zucchini Yellow Resistance Polymorphism (ZYRP), was identified that is linked to the ZYMV-FL resistance gene and a SCAR marker was generated. These markers were used for marker-assisted selection (MAS) to generate a BC2F2 population derived from the donor parent PI 595203 and the recurrent watermelon cultivar Charleston Gray. Progeny from the BC2F2 population exhibited resistance when in possession of homozygous allele from PI 595203. Loss of resistance was seen in resistant BC2F2 plants when compared to the resistance from PI 595203. These newly identified markers are linked to ZYMV-FL resistance and can be useful in a MAS breeding program not only in watermelon, but in other cucurbits.