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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL OF PATHOGENIC AND SPOILAGE BACTERIA ON RED MEAT Title: Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States

Authors
item Kalchayanand, Norasak
item Arthur, Terrance
item Bosilevac, Joseph
item Harhay, Dayna
item Shackelford, Steven
item Wells, James
item Wheeler, Tommy
item Koohmaraie, Mohammad - FORMER ARS EMPLOYEE

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 10, 2009
Publication Date: June 6, 2009
Citation: Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wells, J., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States [abstract]. Institute of Food Technologists (IFT), Division Food Microbiology, Session 026-15, p. 82.

Interpretive Summary: No interpretive summary is required.

Technical Abstract: Clostridium difficile-associated disease has recently increased in both illness and relapse rates in North American and European countries. This increase has been attributed to the emergence of a toxigenic strain designated as North America pulsed-field gel electrophoresis type 1 or NAP-1. The NAP-1 strain has been isolated from calves’ feces, as well as retail ground beef in Canada, leading to a speculation of illnesses from consumption of contaminated meat products. However, information on C. difficile associated with U.S. beef cattle during processing and in commercially produced ground beef is limited. In order to determine this missing information, samples from various steps during beef production were collected. Each of 525 hides, pre-evisceration, and post-intervention carcasses, and 1,048 commercial ground beef samples were collected across the United States. No C. difficile was detected on pre-evisceration and post-intervention carcasses, or in commercially produced ground beef. The prevalence of C. difficile on hides was 3.2%. A total of 18 isolates from hides were phenotypically and genetically characterized. None of the isolates was identified as NAP-1 strain. The results of this study establish a baseline for prevalence of C. difficile in the U.S. beef processing industry, which has a low risk associated with this pathogen.

Last Modified: 12/22/2014
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