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United States Department of Agriculture

Agricultural Research Service

Research Project: Enhancing Resistance to Diseases and Abiotic Stresses Alfalfa

Location: Vegetable and Forage Crops Production Research

Title: A major QTL is associated with resistance to curly top virus in common bean (Phaseolus vulgaris) landrace G122

Authors
item Larsen, Richard
item MIKLAS, PHILLIP
item Kurowski, C - HARRIS MORAN SEED CO.

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: April 24, 2008
Publication Date: June 1, 2008
Citation: Larsen, R.C., Miklas, P.N., Kurowski, C. 2008. A major QTL is associated with resistance to curly top virus in common bean (Phaseolus vulgaris) landrace G122. Phytopathology. 98:S87.

Technical Abstract: Curly top virus (CTV) is a devastating disease of common bean in the Western U.S. Genetic resistance provides effective control but can be difficult to discern in early generations. Two populations of F5:7 RILs were derived from separate F1 seeds from a cross between the G122 landrace from India known as ‘Jatu Rong’ (CTV-resistant) and Taylor Horticultural (CTV-susceptible). The A and B populations comprising 98 individuals in total were evaluated for reaction to CTV in two field experiments over two years, and were screened for potential markers linked to resistance using a population of RAPD primers. Five dominant RAPD markers derived from G122 and completely linked with each other were associated with a major effect QTL that exhibited stable expression across years and populations. The phenotypic variation explained by the QTL in Population A (43.8%) was greater than in Population B (21.9%). Three additional linked RAPD markers detected a QTL with minor effect but in Population A only. Two of the three markers were polymorphic in the common bean BAT/JALO core mapping population enabling integration of the QTL to linkage group B6. G122 appears to possess resistance independent of the Bct-1 gene conferring resistance to CTV as determined by allelism tests. These findings combined with location of the major QTL on B6, indicate that G122 possesses novel resistance to CTV.

Last Modified: 7/28/2014
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