Author
Melouk, Hassan | |
BROWN, M - ENT & PLP, OK STATE UNIV | |
JU, H - CORNELL UNIVERSITY | |
HUNGER, R - ENT&PLP, OK STATE UNIV | |
CONWAY, K - ENT&PLP, OK STATE UNIV |
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 5/1/2008 Publication Date: 7/27/2008 Citation: Melouk, H.A., Brown, M., Ju, H.J., Hunger, R., Conway, K. 2008. Effect of water potential on sclerotial production by Sclerotinia sclerotiorum in a culture medium [abstract]. Phytopathology 98(6):S104. Available: http://www.apsnet.org/meetings/2008/abstracts/a08ma535.htm. Interpretive Summary: Technical Abstract: Sclerotinia sclerotiorum causes Sclerotinia blight on peanut. Potato dextrose agar medium was prepared and adjusted to various water potentials (-0.4 to -3.4 MPa) using NaCl. Petri plates (9-cm dia) each containing 15 ml of medium were inoculated with a 4-mm agar plug of S. sclerotiorum. Plates were incubated at 25 C in darkness for 3 weeks, and then were allowed to dry by un-covering the plates for 2 weeks at 24+2 C. Sclerotia were removed from the semi-dry medium by using a camel hair brush. Sclerotial mass (g) from each plate was determined. Sclerotial weights from cultures grown at -0.9, -1.4, -1.9, -2.4, and -3.4 MPa were significantly (P=0.05) increased from 3 to 8 fold when compared with those grown at -0.4 MPa. Data from this study suggest that water stress enhances the ability of S. sclerotiorum to produce more sclerotia for its survival. |