HANDLING AND TRANSPORT STRESS INTERACTIONS WITH PATHOGEN BIOLOGY IN SWINE AND CATTLE
Location: Livestock Behavior Research
Title: Intestinal microbial affects of yeast products on weaned and transport stressed pigs
Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: March 28, 2008
Publication Date: July 7, 2008
Citation: Weedman, S., Rostagno, M.H., Patterson, J., Kiess, A., Eicher, S.D. 2008. Intestinal microbial affects of yeast products on weaned and transport stressed pigs [abstract]. Journal of Animal Science. 86, E-Suppl. 2:25.
Study objectives were to determine effects of a commercially available yeast product (XPC, Diamond-V Mills) and stress of transportation on total Enterobacteriaceae, Escherichia coli, coliforms, and Lactobacilli populations in the intestine of weaning pigs. In a RCB design with a 2 x 2 factorial arrangement of yeast (Y) and transport (T), 54 pigs were used (n=12 per treatment and 6 baseline pigs). XPC was delivered orally in milk to provide 0.1g/kg of BW and controls (C) received milk only from d 4 to 21 (weaning). Pigs were transported (n=24) or moved (n=24) to nursery housing then supplemented with 0.2% XPC or a grain blank in wk 1 and 2 diets. Samples collected on d1 pre- and d 1, 4, 7, and 14 post-transport included mesenteric lymph node (MLN) and jejunal (Jj), ileal, and cecal contents. Data in parentheses are for YT, Y, CT, and C, treatments respectively. Jejunal coliforms and Enterobacteriaceae; ileal Lactobacilli, coliforms, E. coli, and Enterobacteriaceae; cecal Lactobacilli, coliforms, E. coli, and Enterobacteriaceae were affected (P<0.05) by sampling day. Mean bacterial counts (cfu/g of sample) by d across treatments for Lactobacilli (cfu/g of sample) for d 1, 4, 7, and 14 were 8.0, 8.8, 8.8, and 7.6 for jejunal samples; 8.3, 8.8, 9.1, and 8.1 for ileal samples; and 8.4, 9.2, 8.9, and 8.2 for cecal samples. Coliforms (cfu/g of sample) for d 1, 4, 7, and 14 were 8.5, 9.3, 9.8, and 9.8 for jejunal samples; 8.5 9.8, 9.8, and 9.8 for ileal samples; and 8.0, 9.2, 9.5, and 9.8 for cecal samples. E. coli (cfu/g of samples) for d 1, 4, 7, and 14 were 6.3, 5.0, 5.4, and 5.0 for jejunal samples; 7.5, 6.2, 6.4, and 5.2 for ileal samples; and 7.1, 6.7, 6.1, and 5.6 for cecal samples. Transport by d (P=0.01) and transport by yeast (P=0.10) interactions were detected such that pigs had more E. coli in the cecum on d 1 post-transport (7.6ab, 6.11b, 7.9a, 6.6ab) than on d 7 (5.9b, 7.5a, 5.2b, 6.0ab). Yeast treatment stabilized coliforms and Enterobacteriaceae counts. Day 1 jejunal coliforms and Enterobacteriaceae counts were equivalent within respective treatments and were greatest (P<0.01) in CT (8.4b, 7.8b, 9.8a, 8.1b). Only one Y pig had Salmonella recovered from MLN on d 7 compared to 3 in all other treatments (P=0.07). Data show transport effects on intestinal microbial concentrations and modulation by the yeast product. These results will be used by yeast product companies and feed companies when deciding the efficacy of inclusion of yeast products to reduce effects of stressors.