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ARS Home » Research » Publications at this Location » Publication #221463
Title: Identification and mapping QTL for high-temperature adult-plant resistance to stripe rust in winter wheat (Triticum aestivum L.) cultivar ‘Stephens’

Author
item SANTRA, D - WASHINGTON STATE UNIV
item Chen, Xianming
item SANTRA, M - WASHINGTON STATE UNIV
item Garland-Campbell, Kimberly
item KIDWELL, K - WASHINGTON STATE UNIV

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/24/2008
Publication Date: 6/27/2008
Citation: Santra, D.K., Chen, X., Santra, M., Garland Campbell, K.A., Kidwell, K.K. 2008. Identification and mapping QTL for high-temperature adult-plant resistance to stripe rust in winter wheat (Triticum aestivum L.) cultivar ‘Stephens’. TAG http://dx.doi.org/10.1007/s0122-008-0820-5

Interpretive Summary: High-temperature adult-plant (HTAP) resistance from the winter wheat cultivar ‘Stephens’ has protected wheat crops from stripe rust for 30 years. The objectives of this study were to identify major quantitative genes for HTAP resistance in Stephens through genetic linkage analysis and identify DNA markers linked to the genes for use in marker-assisted breeding. In this study, we used mapping populations consisted of 101 recombinant inbred lines from a cross between resistant Stephens and susceptible ‘Michigan Amber’. The F5, F6, and F7 lines were evaluated at Pullman in 1996, 1997, and 1998, respectively, and the F8 lines were evaluated at Mt. Vernon in 2005 for stripe rust resistance. Stripe rust reaction and severity data were recorded for each of the progeny and the parental lines three times during each of the growing seasons. The 101 lines of the various generations were evaluated with 250 resistance gene analog polymorphism, 245 simple sequence repeat, and 1 microsatellite molecular markers for constructing genetic linkage maps. Two genes associated with the HTAP resistance in Stephens were identified; however, variation in the level of significance of associations was detected among locations and across years. Both genes were detected on chromosome 6B, one on the short arm and one on the log arm. The molecular markers associated with the HTAP resistance genes were identified, which will be used in marker-assisted breeding programs to efficiently incorporate HTAP resistance from Stephens into new cultivars.

Technical Abstract: High-temperature adult-plant (HTAP) resistance from the winter wheat (Triticum aestivum) cultivar ‘Stephens’ has protected wheat crops from stripe rust caused by Puccinia striiformis f. sp. tritici for 30 years. The objectives of this study were to identify major quantitative trait loci (QTL) for HTAP resistance in Stephens through genetic linkage analysis and identify DNA markers linked to the QTL for use in marker-assisted breeding. Mapping populations consisted of 101 recombinant inbred lines (RILs) from ‘Stephens’ (resistant) x ‘Michigan Amber’ (susceptible). F5, F6 and F7 RILs were evaluated at Pullman in 1996, 1997 and 1998, respectively, whereas F8 derivatives were evaluated at Mt. Vernon in 2005. Infection type (IT) and disease severity (DS) were recorded for each RIL three times during each of the growing seasons and area under the disease progress curve (AUDPC) values for each RIL were calculated using the DS data. The 101 RILs were evaluated with 250 resistance gene analog polymorphism (RGAP), 245 simple sequence repeat (SSR) and 1 sequence tagged site (STS) markers for genetic linkage map construction. Two QTL associated with HTAP resistance in Stephens were identified; however, variation in the level of significance of associations was detected among locations and across years. QYrst.wgp-6BS (LOD = 3.89-6.83, R2 = 0.32-0.45, P < 0.0001) and QYrst.wgp-6BL (LOD = 1.4-8.31, R2 = 0.25-0.43, P < 0.0001) were detected on chromosome 6B within a 3.9 cM region flanked by Xbarc101 and Xbarc136 and a 17.5 cM region flanked by Xgwm132 and Xgdm113, respectively. The molecular markers associated with the QTL for HTAP resistance to stripe rust will be used in marker-assisted breeding programs to efficiently incorporate HTAP resistance into regionally adapted cultivars.