Author
KRAUS, JENNIFER - OREGON STATE UNIVERSITY | |
IOANNIS, TZANETAKIS - OREGON STATE UNIVERISTY | |
PUTNAM, MELODIE - OREGON STATE UNIVERSITY | |
Martin, Robert |
Submitted to: Phytopathology
Publication Type: Abstract Only Publication Acceptance Date: 4/27/2007 Publication Date: 6/1/2007 Citation: Kraus, J., Ioannis, T.E., Putnam, M.L., Martin, R.R. 2007. Sequence analysis of a new Carlavirus that infects Verbena x hybrida. Phytopathology. 97. Available: http://meeting.apsnet.org/cfa/ssPresentationView.cfm?PresentationID=2001& Interpretive Summary: Technical Abstract: A Verbena x hybrida sample cv. ‘Taylor Town Red’ from Illinois showed symptoms of reduced vigor, leaf mottling, and necrosis on older leaves under low light conditions. Double-stranded RNA extracted from this plant was reverse transcribed, cloned and sequenced. Three viruses, Broad bean wilt virus (Comovirus), a previously undescribed potyvirus and a carlavirus with similarities to Poplar mosaic virus (PopMV) were found in the plant. The complete nucleotide sequence of the carlavirus was obtained and is 8,742 nucleotides in length. The nucleotide sequence of the 3’ terminus of the carlavirus was 96.1% identical to the two kilobase partial sequence of Coleus vein necrosis virus (CVNV). The carlavirus from ‘Taylor Town Red’ is therefore a verbena isolate of CVNV, herein referred to as CVNV-V. A reverse transcription polymerase chain reaction protocol has been developed for CVNV-V. The virus was transmitted mechanically to Chenopodium quinoa, Nicotiana benthamiana, and Gomphrena globosa and by the green peach aphid from ’Taylor Town Red’ to uninfected verbenas. |