|Wang, Kai - NANJING AGRICULTURAL UNIV|
|Song, Xianliang - NANJING AGRICULTURAL UNIV|
|Han, Zhiguo - NANJING AGRICULTURAL UNIV|
|Guo, Wangzhen - NANJING AGRICULTURAL UNIV|
|Sun, Jing - NANJING AGRICULTURAL UNIV|
|Pan, Jiaju - NANJING AGRICULTURAL UNIV|
|Zhang, Tianzhen - NANJING AGRICULTURAL UNIV|
Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 18, 2006
Publication Date: April 12, 2006
Citation: Wang, K., Song, X., Han, Z., Guo, W., Yu, J., Sun, J., Pan, J., Kohel, R.J., Zhang, T. 2006. Complete assignment of the G. hirsutum L. chromosomes by BAC-FISH and translocation analyses. Journal of Theoretical and Applied Genetics. 113:73-80. Interpretive Summary: Cotton genetic mapping was partial until this first report that completes all 26 chromosomes of the upland cotton (Gossypium hirsutum L.). This study resolved six remaining chromosomes by translocation and fluorescence in situ hybridization mapping techniques. The corresponding linkage groups were assigned to the cotton chromosomes. The report essentially helps unify integrated genome mapping for cotton.
Technical Abstract: In cotton, significant progress has been made in genetic map development. By use of a set of EST-SSR markers and an interspecific Gossypium hirsutum × Gossypium barbadense BC1 population, a PCR-based functional molecular genetic map was constructed in our laboratory. The map consists of 907 loci that are assigned onto 20known chromosomes and 6 linkage groups, totally spanning 5,060 cM with an average distance of 5.6 cM between adjacent markers. For six linkage groups that were not assigned to corresponding chromosomes, specific bacterial artificial chromosome (BAC) clones constructed in G. hirsutum acc. TM-1 were identified by screening a cotton library with linkage group-specific SSR markers from our map as BAC-FISH (fluorescence in situ hybridization) probes. By use of cotton BAC-FISH and ten translocation heterozygotes (NT), we assigned the 6 linkage groups (LG) A01, A02, A03, D02, D03 and D08 to chromosomes (chr.) 13, 8, 11, 21, 24 and 19, respectively. The 13 pairs of homeologous chromosomes were established and a new chromosome nomenclature of tetraploid cotton proposed. A set of chromosome specific-SSR markers were also presented. This map and these data provide a set of powerful tools and also a complete framework for cotton genome research.