|Cepica, S - ACAD OF SCI, CZECH REP|
|Masopust, M - ACAD OF SCI, CZECH REP|
|Knoll, A - ACAD OF SCI, CZECH REP|
|Bartenschlager, H - UNIV HOHENHEIM, GERMANY|
|Yerle, M - INRA, TOLOSAN, FRANCE|
|Gelderman, H. - UNIV HOHENHEIM, GERMANY|
Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 1, 2006
Publication Date: November 1, 2006
Citation: Cepica, S., Masopust, M., Knoll, A., Bartenschlager, H., Yerle, M., Rohrer, G.A., Gelderman, H. 2006. Linkage and RH mapping of 10 genes to a QTL region for fatness and muscling traits on pig chromosome X. Animal Genetics. 37:603-604. Interpretive Summary: In this study 10 genes located on human X chromosome similar to a region affecting fatness and body conformation traits in pigs were mapped in the pig genome. This study was conducted to determine the order of genes on the pig X chromosome relative to the human X chromosome for this important region. In addition, allele frequencies were estimated for eight breeds of pigs for eight of these markers. Alignment of gene order obtained by our mapping in the pig with gene order on human chromosome X showed only a single inversion of two closely linked genes. These results indicate that the gene order of pig X chromosome and human X chromosome for the studied region are nearly identical. This will permit selection of positional candidate genes for future studies from the human genome that may affect body composition in pigs.
Technical Abstract: In this study 10 genes located on human chromosome region Xq13.1 - Xq24 homologous to a QTL region for fatness and body conformation traits were linkage and RH mapped in the pig. PCR primers for amplification of porcine genomic DNAs were designed from orthologous human or porcine (HTR2C) sequences. Mapping of nine genes was performed by amplifying gene markers, across the porcine 118 IMpRH panel and analyses carried out using IMpRH server. PCR- RFLP assays were prepared for SNPs detected in seven genes. These SNPs were genotyped in 69 unrelated animals of 8 breeds for estimation of allele frequencies and in all animals of studied mapping families. Seven genes (RPS4X, XIST, POU3F4, NOX1, CENPI, ACSL, CAPN6, PAK3 and HTR2C) were linkage mapped to the porcine chromosome region SW259 – SW1943 on the USDA-MARC linkage map and eight genes (RPS4X, XIST, POU3F4, NOX1, CENPI, SERPINA7, ACSL, CAPN6, PAK3 and HTR2C) on the University Hohenheim linkage maps. Seven genes were newly linkage mapped to the chromosome X region SW259 - SW1943 harboring QTLs for fatness and muscling traits. Four genes belong to a region with a low recombination rate and order could not be determined by linkage analysis. To support gene order obtained by linkage mapping, nine genes (two of them NOX1 and CENPI were not linkage mapped) were also mapped on the RH panel. Alignment of our gene order obtained by linkage and RH mapping with gene order on human chromosome X showed only a single inversion of porcine genes CAPN6 and PAK3.