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United States Department of Agriculture

Agricultural Research Service

Research Project: PATHOGEN REDUCTION AND OPTIMIZATION OF WATER USAGE IN POULTRY PROCESSING OPERATIONS

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Title: Comparison of Growth of Campylobacteriae on Media Supplemented with Organic Acids and on Commerically Available Media

Author
item Hinton, Jr, Arthur

Submitted to: Annual Meeting of the Institute of Food Technologists
Publication Type: Abstract Only
Publication Acceptance Date: April 13, 2006
Publication Date: June 24, 2006
Citation: Hinton Jr, A. 2006. Comparison of growth of campylobacteriae on media supplemented with organic acids and on commerically available media [abstract]. Annual Meeting of the Institute of Food Technologists. #039H-21.

Technical Abstract: Media formulated for culturing Campylobacter must contain growth factors that support growth of these bacteria. Recent research has indicated that Campylobacter can grow in basal medium supplemented with organic acids. In the present study, growth of Campylobacter in broth and agar media supplemented with organic acids was compared to Campylobacter growth on commercially available media that is not supplemented with these acids. Absorbance of Campylobacter cultures grown in basal broth supplemented with fumaric, lactic, malic, and succinic acids (BB + OA) was compared to absorbance of cultures grown in Brucella broth, Mueller-Hinton broth, and Fluid Thioglycollate medium. Results showed that growth of 10 of 15 Campylobacter isolates was significantly greater in BB + OA than in each commercial broth media, while there was no significant difference in growth of 5 Campylobacter isolates in BB + OA and in commercial media. Additionally, the number of cfu/ml of Campylobacter recovered on agar media composed of BB + OA with agar and hemin (BA + OA + hemin) and was compared to the number of bacteria recovered on Blood Agar. Results indicated that there was no significant difference in the number of cfu’s recovered on BA + OA + hemin or Blood Agar. Findings indicated that media supplemented with organic acids may be used as alternatives to some commercially available media for growing and maintaining Campylobacter cultures.

Last Modified: 12/27/2014
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