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United States Department of Agriculture

Agricultural Research Service

Title: Specific Stylet Activities by Sharpshooters Are Involved in Inoculation of Xylella Fastidiosa

item Backus, Elaine

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 25, 2006
Publication Date: March 17, 2006
Citation: Backus, E.A., Joost, P.H., Yan, F. 2006. Specific stylet activities by sharpshooters are involved in inoculation of Xylella fastidiosa. [abstract]. AgBiotech 2006 A Central Calfornia Agricultural Biotechnology Conference p.23.

Technical Abstract: Our work seeks to understand the stylet penetration behaviors of the glassy-winged sharpshooter (GWSS), and how they interact with Xylella fastidiosa (Xf) to facilitate transmission to grapevine. We use videotaping of feeding on transparent diets, combined with electrical penetration graph (EPG) monitoring to identify most details of feeding. Previous studies in the Backus lab found that the B1, C and N waveforms are associated with inoculation of Xf into susceptible grape plants. This study showed that A1 represents the primary formation of the salivary sheath, B1 represents sheath salivation, stylet tip fluttering and probably tasting, and B2 represents stylet sawing through the hardened sheath or tough plant material. It is particularly interesting that the B1spikelet burst is dispersed intermittently throughout other pathway waveforms, e.g. between peaks of A1, as well as in continuous durations by itself. This dispersion, plus last year's research finding that B1 was the only pathway waveform associated with Xf inoculation, suggest that the spikelet bursts might represent precibarial valve movement, an important component of a hypothesized inoculation behavior for Xf.

Last Modified: 4/22/2015
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