Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: January 1, 2007
Publication Date: November 1, 2007
Citation: Brunelle, B.W., Nicholson, T.L. 2007. Microarray - based approach for genome-wide survey of nucleotide polymorphisms. In: Bergman, Nicholas H., editor. Methods in Molecular Biology. Totowa, NJ: Humana Press. p. 267-280. Technical Abstract: DNA microarrays can be used to detect polymorphic loci in addition to identifying genes or regions that are absent within a genome. A survey such as this offers greater insight into the level of diversification within a species or population. This is especially useful in organisms that have near-identical genomic content, but vary in phenotype, as the identification of such polymorphic loci can lead to the characterization of genes linked to unique biological attributes. Here we describe a competitive hybridization assay using DNA microarrays as a comparative genomics tool to identify nucleotide polymorphisms among the closely related strains of Chlamydia trachomatis.