|Xie, J - UA RREC|
|Moldenhauer, K - UA RREC|
|Gibbons, J - UA RREC|
Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: January 1, 2006
Publication Date: February 15, 2006
Citation: Jia, Y., Rutger, J.N., Xie, J., Lin, M.J., Moldenhauer, K., Gibbons, J. 2006. Development of us genetic resources for rice functional genomics. Rice Technical Working Group Meeting Proceedings, February 29-March 2, 2006, Houston, TX. 2006 CDROM. Technical Abstract: To facilitate functional genomics studies and rice improvement, a large mutant population of the US cultivar Katy and several recombinant inbred line populations derived from elite US rice cultivars are being developed. We aim to take 45,000 independent mutant lines to M4 generation using a mid-season US rice cultivar, Katy. Katy, released in 1989 from Arkansas, is a long grain cultivar with good yield potential and milling quality and contains effective and durable blast resistance genes. Katy was subsequently used as one of the major sources for US cultivar development programs and was chosen as a vector for US mutant population development. To date, 6,000 M1 lines induced by ethyl methane sulfonate (EMS), 14,000 M1 lines induced by fast neutrons, and 25,000 M1 lines induced by gamma radiation were collected from 2001 to 2004. Preliminary analysis of M2 seedlings revealed defects in chlorophyll synthesis in approximately 4% of EMS M1 derived lines. A total of 42 blast susceptible mutants derived from fast neutrons and 7 lesion mimic mutants (2 from EMS and 5 from fast neutrons) were identified. Thus far, 20,000 M2 seeds were collected from 6,000 EMS induced M1 and 14,000 fast neutrons induced M1 by single panicle descent. We aim to advance mapping populations to F10 through single seed descent. The mapping populations that currently being advanced are: Lemont X Jasmine 85 (150 F6 and 289 F4); Katy X RU9101001 (237 F9), Raminade Str. 3 X RU9101001 (140 F6, 2000 F2), Cybonnet X C101A51 (200 F6) and Cybonnet X Saber 289 F6. All of these rice genetic materials will be deposited as genetic stocks in the Genetic Stocks-Oryza (GSOR) collection at Dale Bumpers National Rice Research Center (DB NRRC).