Location: Warmwater Aquaculture Research Unit
Title: LOCALIZATION OF XBAI REPETITIVE ELEMENTS TO CHANNEL CATFISH CENTROMERES VIA FLUORESCENCE IN SITU HYBRIDIZATION Authors
Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 31, 2005
Publication Date: June 15, 2005
Citation: Quiniou, S.M., Wolters, W.R., Waldbieser, G.C. 2005. Localization of xbai repetitive elements to channel catfish centromeres via fluorescence in situ hybridization. Animal Genetics 36:353-354. Interpretive Summary: Little is known about the role of repetitive elements in the channel catfish genome. A previously identified class of repetitive DNA sequences, termed XbaI elements, were visualized on channel catfish chromosomes using fluorescence in situ hybridization. The XbaI elements were located primarily at the centromere of every catfish chromosome. This information will assist researchers in the assembly of catfish genetic maps, the integration of genetic and physical genome maps, and the identification of chromosomal regions that control important production traits in catfish.
Technical Abstract: A/T-rich XbaI elements, highly repetitive DNA sequences (321-325 bp) that represent 5-6% of the channel catfish genome were PCR-amplified from genomic DNA and labeled with digoxigenin-dUTP. The labeled DNA was hybridized with metaphase chromosomes and detected using a FITC-labeled anti-digoxigenin antibody. Fluorescent signal was detected at the centromere of every chromosome pair.