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United States Department of Agriculture

Agricultural Research Service

Title: In Vivo Persistence of Mycoplasma Gallisepticum Vaccine Strain 6/85 Singly and Following Challence by Mycoplasma Gallisepticum F-Strain

Authors
item Evans, Jeff
item Collier, Stephanie
item Pharr, G - MISSISSIPPI STATE UNIV.
item Branton, Scott

Submitted to: Southern Poultry Science Society Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 30, 2004
Publication Date: January 15, 2005
Citation: Evans, J.D., Collier, S.D., Pharr, G.T., Branton, S.L. 2005. In vivo persistence of mycoplasma gallisepticum vaccine strain 6/85 singly and following challence by mycoplasma gallisepticum f-strain. Southern Poultry Science Society Meeting. Abstract 84. p. 21.

Technical Abstract: Within the layer industry, commercially available attenuated strains of Mycoplasma gallisepticum (MG) are commonly used to control MG-induced mycoplasmosis. Strain 6/85 is a commonly utilized vaccine strain which has been demonstrated to be safe due to reduced pathogenicity and transmissibility. To further examine the protection afforded by 6/85, the persistence of the strain was monitored in 120 commercial layer chickens housed in biological isolation units (10 layer hens/unit) divided among 3 treatment groups: (1) commercial layer hens vaccinated with 6/85 at 10 wks of age (n=40); (2) commercial layer hens vaccinated with 6/85 at 10 wks of age and challenged with the F strain of MG (FMG) at 22 wks of age (n=40); and commercial layer hens vaccinated with 6/85 at 10 wks of age and challenged with FMG at 45 wks of age (n=40). The in vivo persistence of 6/85 was determined via culturing swabs of the choanal cleft and subsequent MG-specific pvpA PCR. Sampling included preliminary random sampling of 25% of the layer hens of each treatment group at 48 wks of age and subsequent sampling of all study participants at 59 wks of age. Preliminary results demonstrated 6/85 survivability in 10%, 30%, and 10% of layer hens vaccinated with 6/85 at 10 wks singly, layer hens vaccinated with 6/85 at 10 wks of age and FMG at 22 wks of age, and layer hens vaccinated with 6/85 at 10 wks of age and FMG at 45 wks of age, respectively.

Last Modified: 9/23/2014
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