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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #167973

Title: STRAWBERRY NECROTIC SHOCK VIRUS: A NEW VIRUS PREVIOUSLY THOUGHT TO BE TOBACCO STREAK VIRUS

Author
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item Mackey, Isabela
item Martin, Robert

Submitted to: Acta Horticulturae
Publication Type: Proceedings
Publication Acceptance Date: 5/13/2004
Publication Date: 9/25/2004
Citation: Tzanetakis, I.E., Mackey, I.C., Martin, R.R. 2004. Strawberry necrotic shock virus: a new virus previously thought to be tobacco streak virus. Acta Horticulturae. 656: 27-31.

Interpretive Summary: Tobacco streak virus has been reported to have many strains. In this paper, we report that based on sequence analysis of the coat protein gene of 15 isolates of Tobacco streak virus from strawberry and raspberry, these virus isolates form a group of very closely related strains. Using the same sequence data, we show that these strains are very distinct from the type strain of Tobacco streak virus and should be considered a new virus. We propose the name Strawberry necrotic shock virus as a new member of the genus ilarvirus. Using the same sequence data and comparing sequences of other viruses in this genus, we show that Strawberry necrotic shock virus is more distinct from Tobacco streak virus than other viruses are from each other in the genus. This information is critical when carrying out detection assays for this virus in strawberry and raspberry since many of the tests developed for the type strain of Tobacco streak virus fail to detect Strawberry necrotic shock virus. The reverse is also true in that tests that we developed for Strawberry necrotic shock virus do not detect Tobacco streak virus.

Technical Abstract: Tobacco streak virus (TSV) has a wide host range that exceeds 80 species (Fulton, 1948). Most of the efforts carried out previously comparing TSV isolates was based on immunological relations between them. The isolates of the virus from Fragaria and Rubus have been considered very closely related, if not identical, while they are distinct from isolates of other hosts. While trying to characterize the viruses associated with pallidosis disease, we cloned part of the genome of a virus with homology to TSV, but distinct enough to be considered a unique virus and not an isolate of TSV. Here we report the complete sequence of RNA 3 of the virus from a strawberry isolate as well as the sequence of the coat protein gene from an additional nine strawberry as well as five Rubus isolates. The data suggest that Fragaria and Rubus are infected with a virus closely related to TSV, designated as Strawberry necrotic shock virus from the name given by Frasier et al. in the first report of the virus infecting strawberry. We were unable to acquire any amplicons utilizing reverse transcription-polymerase chain reaction with primers derived from the published sequence for TSV, an indication that strawberry and Rubus may not be hosts for TSV.