|Kamenova, Ivanka - USDA, ARS, USHRL|
|Lewandowski, Dennis - UNIV. OF FLORIDA|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2004
Publication Date: June 1, 2004
Citation: Kamenova, I., Rosskopf, E.N., Lewandowski, D., Adkins, S.T. 2004. Characterization of a tobamovirus from tropical soda apple. Phytopathology. 94:S48. Technical Abstract: Foliar symptoms suggestive of virus infection were recently observed on the noxious weed, tropical soda apple (Solanum viarum), in Florida. An agent was mechanically transmitted to Nicotiana benthamiana and virions were isolated from systemically infected leaves. Rod-shaped particles ~300 nm in length were observed in the purified preparations by electron microscopy. The host range determined by mechanical inoculation with purified virions included predominantly plants from the Solanaceae. Purified virions reacted both with IgG specific for Tobacco mosaic virus and also with IgG that recognizes TMV and related tobamoviruses (including Tomato mosaic virus) in indirect enzyme-linked immunosorbent assay. Sequence comparisons of reverse transcription-polymerase chain reaction products amplified from total RNA of infected N. benthamiana using primers specific for the coat protein (CP) of Pepper mild mottle virus (PMMoV) indicated that the CP sequence of the isolated virus was most closely related to PMMoV, with CP nucleotide and amino acid identities of 80 and 83%, respectively.