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ARS Home » Research » Publications at this Location » Publication #161569
Title: THE IMMUNIZATION OF MICE BY FEEDING OF FRUITS OF TRANSGENIC TOMATO CONTAINING THE INTEGRATED GENE TBI-HBS ENCODING THE SYNTHESIS OF ANTIGENIC PROTEIN EPITOPES OF HEPATITIS B VIRUS AND HIV

Author
item SALYAEV, R - IRKUTSK RUSSIA
item REKOSLAVSKAYA, N - IRKUTSK RUSSIA
item POSDNYAKOV, S - NOVOSIBIRSK RUSSIA
item RYZHOVA, T - NOVOSIBIRSK RUSSIA
item NESTEROV, A - NOVOSIBIRSK RUSSIA
item SUMTZOVA, V - IRKUTSK RUSSIA
item PAKOVA, N - IRKUTSK RUSSIA
item MISHUTINA, U - IRKUTSK RUSSIA
item KOPYTINA, T - IRKUTSK RUSSIA
item Hammond, Rosemarie
item SHCHELKUNOV, S - NOVOSIBIRSK RUSSIA

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 3/1/2004
Publication Date: 3/21/2004
Citation: Salyaev, R.K., Rekoslavskaya, N.I., Posdnyakov, S.G., Ryzhova, T.S., Nesterov, A.E., Sumtzova, V.M., Pakova, N.V., Mishutina, U.O., Kopytina, T.V., Hammond, R., Shchelkunov, S.N. 2004. The immunization of mice by feeding of fruits of transgenic tomato containing the integrated gene tbi-hbs encoding the synthesis of antigenic protein epitopes of hepatitis b virus and hiv. Meeting Proceedings. Vol. 10. Suppl. 1, p. S49.

Interpretive Summary:

Technical Abstract: The synthetic chimeric gene TBI-HBS, encoding the immunogenic ENV and GAG epitopes of HIV-1 (immunogens of T- and B-lymphocytes) and the surface protein (HBsAg) of the hepatitis B virus (HBV), was introduced into tomato plants var. Ventura by means of the agrobacterial vector pBINp35STBI-HBS, and transgenic tomato plants with the integrated gene TBI-HBS were generated. The integration and the expression of the target gene TBI-HBS were confirmed by PCR, Southern and Northern blot hybridizations.The synthesis of chimeric TBI-HBS protein in fruits of transgenic tomato plants was verified by immunoassays. The fruits of transgenic tomato of T1 generation were fed to experimental mice for a period of one week. On the 14th and 28th days post-feeding, high levels of antibodies specific to the antigenic proteins of HBV were found in the serum of tested experimental mice. HBV-specific antibodies were also found in feces of tested experimental mice. There were no HBV-specific antibodies in the control group of mice. Therefore, it was found that the antigens HBsAg (HBV) and TBI (HIV-1) were synthesized in fruits of transgenic tomato as a result of the integration of the construct pBINp35STBI-HBS and the expression of the chimeric protein TBI-HBS was at a level that was able to induce the formation of the immunogenic response in mice after the oral delivery of the edible vaccine.