Technical Abstract: Numbers of Campylobacter detected on broiler carcasses decrease due to scalding but rebound during defeathering. Earlier research indicated that escape of gut contents during defeathering is an important contributor to the increase in Campylobacter numbers while aerosols created by feather picking machines are not. The objective of this study was to determine if contact with surfaces in naturally contaminated commercial feather picking machines contributes to the increased counts of Campylobacter on broiler carcasses. Fully processed chilled carcasses with low numbers of Campylobacter were used as a platform to measure numbers after picking. Chilled carcasses were hung on shackles and allowed to proceed through three empty feather picking machines in a commercial processing plant that had been previously soiled by about 20,000 broilers. In four of five replications, although Campylobacter numbers increased on plant run control birds due to defeathering, no increase in Campylobacter numbers was noted on chilled carcasses due to passage through the pickers. In one replication however, a significant increase was observed. It is possible for contact with soiled surfaces within feather picking machines to increase Campylobacter numbers detected on broiler carcasses. However, overall these data suggest that naturally contaminated surfaces of feather picking machines are not a primary cause of the reported increase in Campylobacter numbers during defeathering.