|Kroppenstedt, R - DSMZ, GERMANY|
Submitted to: American Society for Microbiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 27, 2004
Publication Date: N/A
Technical Abstract: During the course of a 16S ribosomal RNA sequence phylogenetic evaluation of putative Glycomyces strains held in the ARS Culture Collection, Strain NRRL B-16338 was discovered that is phylogenetically near to the genus Glycomyces cluster, but apparently is not a member of this or any of the other currently described actinomycete genera. The strain was subjected to a polyphasic study using standard methods for chemotaxonomic, morphological and physiological evaluation and exhibited chemotaxonomic characteristics distinct from Glycomyces in spite of having 16S rRNA sequence homology of 92% with the described species of this genus. Sugars determined from capillary gas chromatography of alditol acetates from whole cell hydrolysates of NRRL B-16338 consisted of ribose and inositol, with traces of arabinose and mannose. The phospholipids observed were phosphatidylglycerol and diphosphatidyl glycerol, and menaquinones content consisted of MK-10(H4), MK-10(H6), MK-11(H4), and MK-11(H6). A significant quantity (14.5%) of 17:0 anteiso 2-hydroxy fatty acid was observed in the fatty acid profile of this strain. These characteristics clearly differentiate NRRL B-16338 from members of the genus Glycomyces and it is proposed that the strain represents a new genus within the order Actinomycetales to be called Stackebrandtia gen. nov.. The strain was originally isolated from a soil sample collected in Puerto Rico and it is proposed that the type species of the genus should be named Stackebrandtia puertoricensis.