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United States Department of Agriculture

Agricultural Research Service

Title: Simple Sequence Repeat Markers for Botanical Varieties of Cultivated Peanut (Arachis Hypogaea L.)

Authors
item He, Guohae - TUSKEGEE UNIV/TUSKEGEE,AL
item Meng, Ronghua - TUSKEGEE UNIV/TUSKEGEE,AL
item Gao, Hui - TUSKEGEE UNIV/TUSKEGEE,AL
item Guo, Baozhu
item Gao, Guoqing - GUANGXI/NANNING,CHINA
item Harrison, Melanie
item Pittman, Roy
item Prakash, C - TUSKEGEE UNIV/TUSKEGEE,AL

Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 12, 2004
Publication Date: January 20, 2005
Citation: He, G., Meng, R., Gao, H., Guo, B., Gao, G., Newman, M., Pittman, R. N., Prakash, C. S. 2005. Simple sequence repeat markers for botanical varieties of cultivated peanut (Arachis hypogaea L.). Euphytica. 142:131-136.

Interpretive Summary: Peanut (Arachis hypogaea L.) is an important crop grown worldwide for its oil and protein sources. Cultivated peanut has six botanical varieties in which each has its specific morphological characteristics. It is difficult to identify true hybrids in peanut breeding with intercross between two subspecies. Therefore, it would be best to use molecular markers coupled with morphological characteristics to detect hybrids in breeding programs. Identification of DNA markers associated with botanical varieties would be useful in plant genotyping, germplasm management, and breeding. In this study, we used 48 cultivated accessions representing six botanical varieties to identify botanical variety-specific markers. One hundred thirty peanut simple sequence repeat (SSR) primers were screened on 24 cultivated accessions. Thirty-eight primers detecting DNA polymorphism among accessions were used for SSR analysis, in which 29 were developed from microsatellite-enriched library, and 9 were identified from peanut expressed sequence tags (ESTs). Eight peanut SSR markers were related specific to botanical varieties. Three markers derived from peanut ESTs were associated with functional genes. As botanical varieties have different morphological traits and belong to different subspecies, these markers might be associated with the expression of morphological traits and could be used in breeding programs.

Technical Abstract: Cultivated peanut (Arachis hypogaea L.) consists of six botanical varieties. Identification of DNA markers associated with botanical varieties would be useful in plant genotyping, germplasm management, and breeding. Forty-eight cultivated accessions representing six botanical varieties were used to identify botanical variety-specific markers. One hundred thirty peanut simple sequence repeat (SSR) primers were previously screened on 24 cultivated accessions. Thirty-eight primers detecting DNA polymorphism among accessions were used for SSR analysis, in which 29 were developed from microsatellite-enriched library, and 9 were identified from peanut expressed sequence tags (ESTs). Eight peanut SSR markers were related to botanical varieties. Among them, six SSR markers were specific to botanical varieties fastigiata and vulgaris, one to botanical varieties hypogaea and hirsuta, and one to botanical varieties peruviana, and aequatoriana. Three markers derived from peanut ESTs were associated with functional genes. Multiplex PCR of botanical variety-specific markers could be applied to facilitate efficient genotyping of the peanut lines. As botanical varieties have different morphological traits and belong to different subspecies in A. hypogaea, these markers might be associated with the expression of morphological traits.

Last Modified: 8/1/2014
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