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United States Department of Agriculture

Agricultural Research Service

Title: Zonierung Von Intakten Bakteriophagen

Authors
item Barrangou, R - NCSU
item BREIDT, FREDERICK
item Schroen, D - KENDRO NEWTOWN CT

Submitted to: Git Labor-Fachzeitschrift
Publication Type: Research Notes
Publication Acceptance Date: September 1, 2002
Publication Date: October 1, 2002
Citation: Barrangou, R., Breidt, F., Schroen, D. 2002. Zonierung von intakten bakteriophagen. Git Labor-Fachzeitschrift.4:470-471.

Interpretive Summary: With recent advances in molecular biology, the isolation of bacteriophage continues to increase. The SORVALL Discovery M150 micro-ultracentrifuge allows rapid concentration and purification of bacteriophage in a CsCl step gradient. The pure phage solution obtained via ultracentrifugation can be subsequently used for electron microscopy and protein or nucleic acid analysis. This article originated as Banding of Intact Bacteriophage Using the S100-AT6 Rotor and Discovery M150 Micro-ultracentrifuge and appeared in a Kendro Sorvall Application Brief published by Kendro Laboratory Products, Newton, CT.

Technical Abstract: With recent advances in molecular biology, the isolation of bacteriophage continues to increase. The SORVALL Discovery M150 micro-ultracentrifuge allows rapid concentration and purification of bacteriophage in a CsCl step gradient. The pure phage solution obtained via ultracentrifugation can be subsequently used for electron microscopy and protein or nucleic acid analysis. This article originated as Banding of Intact Bacteriophage Using the S100-AT6 Rotor and Discovery M150 Micro-ultracentrifuge and appeared in a Kendro Sorvall Application Brief published by Kendro Laboratory Products, Newton, CT.

Last Modified: 9/10/2014
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