|Scheets, K - OKLAHOMA STATE UNIVERSITY|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2002
Publication Date: May 10, 2002
Citation: Redinbaugh, M.G., Scheets, K. 2002. Maize necrotic streak virus is most closely related to members of the genus tombusvirus (abstract). Phytopathology. 92:S68. Technical Abstract: Initial reports indicated that Maize necrotic streak virus (MNeSV) is most closely related to viruses in the family Tombusviridae. The two 5' most open reading frames (ORFs) are most simlar to the corresponding tombusvirus proteins, while the 27.4 kDa coat protein (CP) is more closely related to the Tobacco necrosis virus CP. In this study, the complete sequence of MNeSV (4094 nt) was shown to be significantly smaller than the typical 4.7 kb tombusvirus genome. The 122 nt 5' untranslated region (UTR) could fold into a stable T-shaped domain. Overlapping ORFs at the genome's 3' end encoded proteins of 21.4 kDa and 19.0 kDa are similar to the tombusvirus p21 and p19, respectively. The 3' UTR was 196 nt long, and did not contain the small ORF (pX) found in some tombusviruses. Three sgRNAs of 1607, 781 and 190 nt were found. MNeSV vRNA replicates readily in Black Mexican Sweet maize protoplasts facilitating future studies.