ARS | Publication request: SHOOT-TIP MICROGRAFTING FOR RESCUE OR THERAPY OF FIELD-GROWN CITRUS GERMPLASM ACCESSIONS

Submitted to: International Society of Citriculture Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 15, 2000
Publication Date: December 15, 2000
Citation: KRUEGER, R., KAHN, T.L., GUMPF, D.J., BALLANCE, P.M., BIER, O.J., BASH, J.A. SHOOT-TIP MICROGRAFTING FOR RESCUE OR THERAPY OF FIELD-GROWN CITRUS GERMLASM ACCESSIONS. INTERNATIONAL SOCIETY OF CITRICULTURE ABSTRACTS. 2000.

Technical Abstract: Shoot-tip micro-grafting (STG) is a well-established technique for the potential elimination of virus and viroid pathogens from citrus germplasm. It is generally utilized in quarantine and certification programs using small trees maintained under glass or screen. A modified STG procedure utilizing shoot-tips from a declining field-grown tree was used to produce a replacement tree. It had not been possible to propagate the tree by budding or other conventional techniques. Since the source tree subsequently died, the modified STG procedure allowed the rescue of an accession that would otherwise have been lost. An additional benefit was the apparent elimination of a viral pathogen: the source tree had previously tested positive for Citrus Tristeza Virus, while the replacement tree tested negative.