ARS | Publication request: Identification of Molecular Markers Associated with Barley Malt Quality.

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 21, 2000
Publication Date: November 25, 2000
Citation: Garcia, G., Horsley, R.D., Dahleen, L.S., Schwarz, P.B., Green, M.J. 2000. Identification of molecular markers associated with barley malt quality. Agronomy Abstracts, p. 100.

Technical Abstract: Identification of loci controlling grain protein concentration (GPC) in barley, the effect of environmental factors on its expression, and the relationship between GPC and other malt quality traits would advance our knowledge about the genetics of GPC and co-variation with other malt quality traits. Previous research has identified associations between low GPC, and (DP) and kernel color. One hundred four recombinant inbred lines (RIL) were derived from the cross Azure/ND5377. Azure is the high GPC parent and ND5377 is the low GPC parent. The source of low GPC in ND5377 is Karl. The objectives of this study were to: 1) localize the low-GPC loci to chromosomes, 2) to determine the genetic inter-relationship between GPC, DP, and kernel color, and 3) to determine the effect of high temperature during grain filling on the expression of GPC. Chromosome 6H was identified as the putative chromosomal location for GPC, DP, and kernel color. Two RFLP loci and one SSR locus have been identified to be associated with protein, DP, and kernel color. A chromosome map of 6H with loci for GPC, DP, and kernel color will be presented.