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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Characterization of the Acid Phosphatase Exuded from Proteoid Roots of P Stressed White Lupin

Authors
item Miller, Susan - UNIVERSITY OF MINNESOTA
item Liu, Junqi - UNIVERSITY OF MINNESOTA
item Temple, Stephen - UNIVERSITY OF MINNESOTA
item Gilbert, Glena - UNIVERSITY OF MINNESOTA
item Menzhuber, Chris - ST THOMAS UNIVERSITY
item Allan, Deborah - UNIVERSITY OF MINNESOTA
item Vance, Carroll

Submitted to: American Society of Plant Physiologists Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 17, 2000
Publication Date: N/A

Technical Abstract: White lupin when subjected to a low phosphorus (P) environment forms proteoid (cluster) roots. Proteoid roots from P-stressed plants exude organic acids and acid phosphatase (APase) enzyme as a strategy to acquire unavailable P. We have isolated an APase cDNA clone that is highly expressed in P-stressed proteoid roots but not in other tissues. This white elupin APase cDNA clone is 1532 bps in length with an ORF of 1380 bp. The 460 amino acid lupin APase polypeptide shows good similarity with the purple APases of arabidopsis and bean. In order to establish if this APase cDNA encoded the APase enzyme exuded from P-stressed proteoid roots, we purified the exuded APase and determined its N terminal amino acid sequence. Amino acid residues 32-41 of the deduced APase polypeptide were a direct match with the 10 N terminal amino acids of the exuded enzyme. These data demonstrate that we have in fact isolated the cDNA encoding the P- stress induced APase exuded from proteoid roots. The processed, exuded enzyme is comprised of 429 AA with a presequence of 31 AA. PSORT analysis of the 31 AA presequence targets the protein to the outside of the cell with a .82 certainty. Antibodies have been produced to lupin P-stress exuded APase for cellular localization studies. In addition, the 5'- upstream region of the lupin APase gene has been isolated.

Last Modified: 8/27/2014
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