Submitted to: Food and Agricultural Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 6, 2002
Publication Date: December 1, 2002
Citation: Brandon, D.L., Bates, A.H., Binder, R.G., Montague Jr, W.C. 2002. Monoclonal antibody to fenbendazole: utility in residue studies. Food and Agricultural Immunology. 14(4):275-283. Interpretive Summary: Because of concern for the safety of the food supply, regulatory tolerances have been established for residues of veterinary drugs used in animal management. Foods such as milk and beef products are randomly tested for residues, typically by procedures relying on hazardous solvents. This paper describes a method to test for residues of fenbendazole, an anti-worm drug, using a monoclonal antibody-based assay conducted in water solution. This test proved applicable to both liver and milk samples, and is particularly useful for screening samples, most of which fall below regulatory tolerances.
Technical Abstract: A monoclonal antibody-based ELISA was developed for fenbendazole, a widely used benzimidazole anthelmintic, with approved uses in cattle and swine. The antibody was elicited using as hapten 2-succinamido-5(6)-phenylthiobenzimidazole, which was conjugated to bovine serum albumin to produce an immunogen and to horseradish peroxidase, to prepare a labelled ligand. In the milk matrix, the limit of detection was 3 ppb; in bovine liver, 200 ppb. The ELISA method is a simple approach to screen food samples for residues of fenbendazole and febantel (a pro-drug related to fenbendazole) and may also be useful in studying the pharmacokinetics of fenbendazole.