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United States Department of Agriculture

Agricultural Research Service

Title: COMPARISON OF MUCOSAL COMPETITIVE EXCLUSION AND COMPETITIVE EXCLUSION TREATMENT TO REDUCE SALMONELLA AND CAMPYLOBACTER SPP COLONIZATION IN BROILER CHICKENS

Authors
item Stern, Norman
item Cox, Nelson
item Bailey, Joseph
item Berrang, Mark
item Musgrove, Michael

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 26, 2000
Publication Date: February 1, 2001
Citation: Stern, N.J., Cox Jr, N.A., Bailey, J.S., Berrang, M.E., Musgrove, M.T. 2001. Comparison of mucosal competitive exclusion and competitive exclusion treatment to reduce salmonella and campylobacter spp colonization in broiler chickens. Poultry Science. 80(2): 156-160.

Interpretive Summary: Bacteria from the intestinal tracts of adult birds have been used to develop cultures which prevent human pathogens from establishing themselves in the guts of chicks. Aho and others developed a culture to prevent Salmonella from living in chicks while Stern and colleagues developed a culture to prevent Campylobacter colonization of chicks. Using each of these methods, cultures were developed, used to treat chicks, and their abilities to prevent colonization were compared. Cultures grown using the method of Stern and others were better at reducing colonization by both Campylobacter and Salmonella than the cultures produced by the Aho method.

Technical Abstract: Control of Salmonella spp. during growout may help reduce human pathogens on processed broiler carcasses. Use of the "Nurmi concept" has been reduced Salmonella in broiler chicks. Aho et. al, created a competitive exclusion culture (GB) for control of Salmonella spp. while a mucosal competitive exclusion (MCE) culture developed in the United States was originally created to control Campylobacter colonization.(Stern et al. 1995). Major differences of the two patents were the means of collecting cells from the adult chickens' ceca and the media/condition used to grow them. GB and MCE were compared for their ability to reduce both Salmonella and Campylobacter in broiler chick intestines. Nine adult birds (three for each of three trials) were killed and each of the bird's two ceca was used to produce corresponding cultures(one by each for GB and MCE), then given to chicks which were challenged 24 h later with Salmonella and Campylobacter, sacrificed one week later when levels of the pathogens were determined. GB treated birds were significantly more colonized by S. typhimurium (3.97 log 10 cfu/g cecal contents v. 1.25 log10 cfu/g cecal contents)and Campylobacter spp.(6.96 log10 cfu/g cecal contents v. 5.03 log10 cfu/g cecal contents) than those treated with the MCE. These results can be used in an intervention program to reduce chicken colonization by Salmonella and Campylobacter.

Last Modified: 10/31/2014