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United States Department of Agriculture

Agricultural Research Service

Research Project: CONSERVATION, CHARACTERIZATION, AND EVALUATION OF CROP GENETIC RESOURCES AND ASSOCIATED INFORMATION

Location: Plant Genetic Resources Conservation Unit

Title: FAD2 gene mutations significantly alter fatty acid composition in peanuts.

Authors
item Wang, Ming
item Barkley, Noelle
item Chen, Zhenbang -
item Pittman, Roy
item Pederson, Gary

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 24, 2011
Publication Date: January 14, 2012
Citation: Wang, M.L., Barkley, N.L., Chen, Z., Pittman, R.N., Pederson, G.A. 2012. FAD2 gene mutations significantly alter fatty acid composition in peanuts. Plant and Animal Genome Conference. January 14-18, 2012. San Diego, CA. p.459.

Interpretive Summary: Peanut seed fatty acid composition is one of the important parameters to determine the oil quality. A panel of 55 peanut lines was analyzed for fatty acid composition with gas chromatography and also genotyped with SNP markers from the FAD2 genes by real-time PCR. Significant variation in fatty acid composition was identified and the ratio of oleic acid to linoleic acid (O/L) ranged from 1.23 to 56.45. In terms of the FAD2 gene mutation, the assayed lines were classified into four genotypes: wild type (Ol1Ol1Ol2Ol2), single functional homozygous mutation on the A genome (ol1ol1Ol2Ol2), single functional homozygous mutation on the B genome (Ol1Ol1ol2ol2), and a double mutation on both A and B genomes (ol1ol1ol2ol2). Each genotype has a significantly different fatty acid profile. Both FAD2A and FAD2B are involved in the conversion of oleic acid to linoleic acid in peanut. Overall, these results demonstrate the combined power of genetic analysis with biochemical analysis on peanut fatty acid research.

Technical Abstract: A panel of 55 peanut lines was analyzed for fatty acid composition with gas chromatography and also genotyped with SNP markers from the FAD2 genes by real-time PCR. Significant variation in fatty acid composition was identified and the ratio of oleic acid to linoleic acid (O/L) ranged from 1.23 to 56.45. In terms of the FAD2 gene mutation, the assayed lines were classified into four genotypes: wild type (Ol1Ol1Ol2Ol2), single functional homozygous mutation on the A genome (ol1ol1Ol2Ol2), single functional homozygous mutation on the B genome (Ol1Ol1ol2ol2), and a double mutation on both A and B genomes (ol1ol1ol2ol2). Each genotype has a significantly different fatty acid profile. Both FAD2A and FAD2B are involved in the conversion of oleic acid to linoleic acid in peanut. Overall, these results demonstrate the combined power of genetic analysis with biochemical analysis on peanut fatty acid research.

Last Modified: 9/1/2014
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