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United States Department of Agriculture

Agricultural Research Service

Research Project: SYSTEMATICS, GENETIC DIVERSITY ASSESSMENT, AND ACQUISITION OF POTATOES, CARROTS, AND THEIR RELATED WILD RELATIVES Title: Wart (synchytrium endobioticum) resistance in cultivated potato species

Authors
item Antonova, O -
item Hutti, A -
item Shuvalov, O -
item Islamshina, A -
item Afanasenko, O -
item Spooner, David
item Gavrilenko, T -

Submitted to: European Association for Potato Research
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2011
Publication Date: July 24, 2011
Citation: Antonova, O., Hutti, A., Shuvalov, O., Islamshina, A., Afanasenko, O., Spooner, D.M., Gavrilenko, T. 2011. Wart (synchytrium endobioticum) resistance in cultivated potato species [abstract]. European Association for Potato Research. p. 185.

Technical Abstract: Potato wart (Synchytrium endobioticum) is an obligate chytrid fungus parasite. It is included in the list of the quarantine objects in 55 countries. At the end of the nineteenth century, the disease spread outside of its original range in the Andeans of South America. More than 40 pathotypes of the fungus exist, but the most widely distributed is pathotype 1. Crop losses in susceptible cultivars can reach 50-100%. Three dominant genes, or one dominant and two complementary genes are effective against pathotype 1 (D1). It was shown that one dominant gene Sen1 on chromosome XI determined resistance to pathotype 1 (D1) of S. endobioticum. The aim of our study was to characterize wart resistance of a recently published experimental subset (Gavrilenko et al., 2010) from the Russian National cultivated potato collection and to determine whether this resistance is associated with traditional cultivated species taxonomy, with ploidy, with geographic distance, or with molecular markers linked to the gene Sen1 and to select new sources of wart resistance. We also screened them with SCAR-marker NL25 (Gebhardt et al, 2006), which is linked to a gene Sen_1. Of the 90 tested landrace accessions, 39 were extremely resistant (score 1), and 25 were resistant (score 2), and three were extremely susceptible (score 5), distributed among all analyzed cultivated species. There was no correlation of wart resistance to cultivated species taxonomy, ploidy, or geographic distance. Only one accession (resistant) of ssp. andigenum of the above four taxa possessed by the diagnostic fragment NL25-1400 bp. Thus, among landraces we did not observe any correlation between the absence/presence of the diagnostic fragment NL25-1400 bp and the level of resistance to potato wart. We also screened 100 modern varieties with NL25 that were selected from prior screening to be resistant (95 varieties) and susceptable (5 varieties). The marker diagnostic allele NL25-1400 bp was detected in 85 resistant varieties, whereas this fragment was not identified in susceptible breeding varieties. Thus, a strong relationship was observed in breeding varieties between the presence of diagnostic component NL 25-1400 bp and the wart resistance. However, within the diversity of landraces, our work shows a lack of predictive associations of wart resistance and cultivated species taxonomy, ploidy, geographic distance, or the selective utility of the NL25 marker.

Last Modified: 11/25/2014
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