ARS | Publication request: In-Vivo Zinc Metabolism by Isotope Ratio Mass Spectrometry

Submitted to: Encyclopedia of Mass Spectrometry
Publication Type: Book / Chapter
Publication Acceptance Date: June 20, 2010
Publication Date: June 20, 2010
Citation: Lowe, N.M., Woodhouse, L.R. 2010. In-Vivo Zinc Metabolism by Isotope Ratio Mass Spectrometry. Encyclopedia of Mass Spectrometry. 5:1001-1012, 2010.

Technical Abstract: The purpose of this chapter is to highlight some of the methodological and technical issues surrounding the in vivo use of stable isotopes and to provide examples of how such studies have advanced our knowledge of human zinc metabolism. The advantages and disadvantages of the currently available instrumentation for measuring isotope ratios are discussed. General methodological issues concerning preparation of isotopes for administration, and choosing the correct dose is presented and this is followed by an overview of applications, which includes a detailed “case study” that takes the reader through a detailed example describing the practical issues of isotope ratio interpretation. The final section presents some of the most recent advances in the use of stable isotopes to study human zinc metabolism.