SUNFLOWER GERMPLASM DIVERSIFICATION AND CHARACTERIZATION UTILIZING WILD SUNFLOWER SPECIES, CYTOGENETICS, AND APPLIED GENOMICS
Location: Sunflower Research
Title: Chromosome Engineering of T7A.7Lr#1S for the Isolation of New Recombinants and Field Evaluation of T7A.7Lr#1S Chromosome Introgression Hard Winter Wheat Lines for Resistance to FHB and DON
| Friebe, Bernd - |
| Cainong, Joey - |
| Pumphrey, Michael |
| Bockus, William - |
| Gill, Bikram - |
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 11, 2009
Publication Date: December 1, 2009
Citation: Friebe, B., Qi, L.L., Cainong, J., Pumphrey, M.O., Bockus, W.W., Gill, B.S. 2009. Chromosome Engineering of T7A.7Lr#1S for the Isolation of New Recombinants and Field Evaluation of T7A.7Lr#1S Chromosome Introgression Hard Winter Wheat Lines for Resistance to FHB and DON [abstract]. Fusarium Head Blight Forum, December 7-9, 2009, Orlando, Florida. p. 117.
T7AL.7Lr#1S is a genetically compensating wheat-Leymus translocation line (T09) involving wheat chromosome 7AL arm and Leymus racemosus (Lr) chromosome 7Lr#1S arm in CS (Chinese Spring) and was consistently resistant to FHB in greenhouse point-inoculation experiments. The novel FHB resistance gene was designated Fhb3 and resides in the distal region of the short arm of chromosome 7Lr#1. T7AL.7Lr#1S was backcrossed twice to Overley and Jagger and ten lines homozygous for T7AL.7Lr#1S, three in Overley and seven in Jagger background, were evaluated for FHB resistance in a field nursery in Manhattan. All of the translocation lines except 08-183 had significantly lower mean disease ratings compared to their susceptible parent Overley. Unfortunately, the other backcross parent Jagger was not included in the test; however, three of the translocation lines (08-193, 08-189, and 08-184) had significantly lower ratings than Jagalene, which is known to be identical to Jagger in its reaction to FHB. It appears that Fhb3 increased resistance in these entries. Similarly, the same three translocation entries had significantly lower DON levels than those of Overley and Jagalene and were statistically similar to moderately-resistant Truman.
Simultaneously, chromosome engineering was initiated to reduce the genetic linkage drag associated with T7AL.7Lr#1S. Three PCR-based markers, BE586744-STS, BE404728-STS, and BE586111-STS, specific for 7Lr#1S, were developed to expedite marker-assisted selection of recombinants. Upon analysis of 1,118 progeny, three wheat-Leymus recombinants, one proximal (#124) and two distal (#679 and #989), have been isolated in homozygous condition. These lines along with resistant and susceptible controls, as well as 08-193, 08-189, and 08-184, will be evaluated for FHB resistance by single point inoculation method in the greenhouses.
ACKNOWLEDGEMENT AND DISCLAIMER
This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0790-7-075. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.