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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #209908

Title: THE ISOAMYL OXIDASE GENE IN PENICILLIUM GRISEOFULVUM IS PART OF THE PATULIN BIOSYNTHETIC PATHWAY

Author
item Dombrink Kurtzman, Mary Ann

Submitted to: Gordon Research Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/22/2007
Publication Date: 6/22/2007
Citation: Dombrink Kurtzman, M. 2007. The isoamyl oxidase gene in penicillium griseofulvum is part of the patulin biosynthetic pathway [abstract]. Gordon Research Conference Proceedings.

Interpretive Summary:

Technical Abstract: Genes for the patulin biosynthetic pathway are likely to be arranged in a cluster, as is the case for other mycotoxins. GeneWalking was performed to identify genes both upstream and downstream of the isoepoxydon dehydrogenase (idh) gene in Penicillium griseofulvum NRRL 2159A. A gene with high sequence homology to the isoamyl alcohol oxidase (iao) gene was detected downstream of the idh gene. The newly identified gene coded for a secreted protein having a 22 amino acid signal peptide, a FAD-binding domain, and 11 potential N-linked glycosylation sites. Searches in GenBank indicated that Aspergillus terreus, A. oryzae, A. fumigatus and Gibberella zeae also contained genes coding for a putative isoamyl alcohol oxidase. This is the first report of an isoamyl alcohol oxidase gene in Penicillium.