Inhibition of colonic cancer cell proliferation and COX2 by oats avenanthramides (Avns)

Authors: NIE, LIN - TUFTS/HNRCA; WISE, MITCHELL - USDA/ARS CEREAL CROPS; Meydani, Mohsen; COLLINS, F.WILLIAM - EASTERN CEREAL & OS CNTR

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/6/2007
Publication Date: 4/2/2007
Citation: Nie, L., Wise, M., Meydani, M., Collins, F. 2007. Inhibition of colonic cancer cell proliferation and COX2 by oats avenanthramides (Avns). Experimental Biology/The FASEB Journal. 21(5):p. A102.

Interpretive Summary:

Technical Abstract: High intake of whole grain foods is associated with reduced risk of colon cancer, but the mechanism underlying this protection has yet to be elucidated. Avns are polyphenols unique to oats. We have reported that Avns inhibited pro-inflammatory cytokines and vascular smooth muscle cell proliferation. We have also discovered that methyl ester of Avns-C (CH3-Avn-C) has more potency. Chronic inflammation may lead to the increased risk of colon cancer. Here, we report the inhibitory effect of Avns on proliferation and expression of COX2 in colonic cancer cell lines. We found that Avns of oat extract and CH3-Avn-C significantly inhibited proliferation of several colonic cancer cell lines in the magnitude order of HCT116>CaCo2>LS174>HT29. In contrast, they had no effect on the differentiated CaCo2 cells, which represent the normal colonic epithelial cells. TNFalpha-induced COX2 expression and prostaglandin E2 production in HT29 cells were decreased by these compounds. They also suppressed the phosphorylation of the p65 subunit of NF-kappaB in this cell line. Our data suggest for the first time that Avns have anti-inflammatory and anti-proliferative effects on colonic cancer cell lines. Supported by USDA agreement No. 58-1950-9-001.