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ARS Home » Plains Area » Mandan, North Dakota » Northern Great Plains Research Laboratory » Research » Publications at this Location » Publication #195753
Title: EVALUATION OF MILK SOMATIC CELLS AS A SOURCE OF MRNA FOR STUDY OF MAMMARY GLAND LIPOGENSIS IN LACTATING BEEF COWS

Author
item MURRIETA, C - UNIVERSITY OF WYOMING
item Scholljegerdes, Eric
item HESS, BRET - UNIVERSITY OF WYOMING
item RULE, DAVID - UNIVERSITY OF WYOMING
item ENGLE, T - COLORADO STATE UNIVESITY
item HOSSNER, K - COLORADO STATE UNIVERSITY

Submitted to: Western Section of Animal Science Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2005
Publication Date: 7/22/2005
Citation: Murrieta, C.M., Scholljegerdes, E.J., Hess, B.W., Rule, D.C., Engle, T.E., Hossner, K.L. 2005. Evaluation of milk somatic cells as a source of mrna for study of mammary gland lipogensis in lactating beef cows. Western Section of Animal Science Proceedings. Vol. 56:36-39.

Interpretive Summary:

Technical Abstract: Our objective was to compare mRNA levels for acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), lipoprotein lipase (LPL) and stearoyl-CoA desaturase (SCD) extracted from mammary gland and from somatic cell pellets of the milk from each mammary gland. Eighteen primiparous beef cows (BW = 411 ± 24.3 kg.; BCS = 5.25) were fed Foxtail millet hay at 2.13% of BW and either a low-fat control (CON; n = 9) or a cracked high-linoleate (67% 18:2 n-6) safflower seed supplement (LIN; n = 9). Diets were isonitrogenous and isocaloric, and the LIN diet contained 5% of DMI as fat. At slaughter (37 ± 3 d postpartum) mammary tissue was sampled and immediately frozen in liquid N2 before being stored at -80oC. Milk samples were obtained from the same mammary glands and immediately spun at 1,200 ' g to pellet somatic cells. Ribonuclease protection assay was used to quantify the mRNA. Data were analyzed for a 2 ' 2 factorial experiment to test for dietary, tissue and interactive effects. Dietary, tissue, and interactions were not observed for ACC (P = 0.21; 0.91; and 0.45, respectively), FAS (P = 0.32; 0.71; and 0.28, respectively), LPL (P = 0.09; 0.15; and 0.43, respectively), or SCD (P = 0.34; 0.26; and 0.37, respectively). Correlation analysis was performed between mammary tissue and milk somatic cell data within dietary treatment for each mRNA. Within the CON treatment, Pearson correlation coefficients were: ACC, 0.75 (P = 0.02); FAS, 0.69 (P = 0.04); LPL, 0.69 (P = 0.04); and SCD, 0.67 (P = 0.05). Within the LIN treatment, Pearson correlation coefficients were: ACC, 0.85 (P = 0.004); FAS, 0.75 (P = 0.02); LPL, 0.90 (P = 0.001); and SCD, 0.73 (P = 0.03). We conclude that using milk obtained from lactating beef cows can be used as a source of RNA to study regulation of mammary gland lipogenesis.