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United States Department of Agriculture

Agricultural Research Service

Research Project: Rapid and Accurate Diagnostic Identification of Phytoplasmas for Cooperative Agricultural Pest Survey (Caps) Program

Location: Molecular Plant Pathology

Project Number: 1245-22000-282-20
Project Type: Interagency Reimbursable Agreement

Start Date: Mar 01, 2014
End Date: Sep 30, 2014

Objective:
This project is a continuation of a previously funded project whose objectives are to enhance and strengthen pest identification and technology. Specifically this project will improve early detection surveys, a strategy for this goal, by allowing identification at strain/species level for phytoplasmas.

Approach:
ARS will select the best methods to use for plant sample storage and for rapid and reliable extraction protocols for total nucleic acids from leaves. Symptomatic plants will be tested by state or private labs using a phytoplasma nested polymerase chain reaction (PCR). Phytoplasma positive samples will then be analyzed by ARS using molecular tools (Restriction Fragment Length Polymorphism {RFLP} analysis) to determine the specific strain/species present and to distinguish between endemic phytoplasmas and the exotic phytoplasmas that are being surveyed for in early detection surveys. This project will address the significant gap in phytoplasma diagnostic capacity at the strain/species level. This project will also enhance taxonomic capacity for phytoplasmas, and will involve the deployment of molecular diagnostic tools, which are both strategies of this goal area. This project aligns with the diagnostic capacity building category. Specifically this fits within molecular tool development/validation for CAPS national survey targets for phytoplasmas at the species/strain level.

Last Modified: 9/10/2014
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