Project Number: 1902-13000-012-34
Start Date: Apr 15, 2014
End Date: Dec 31, 2016
Select grasses, inoculated and uninoculated, will be planted and grown in a complete block design and fertilized at 0, 25%, 50%, 75% and 100% of the recommended N rate. Field colonization will be assessed by DNA extraction, PCR amplification, and comparison with a positive control derived from previously sequenced G. diazotrophicus culture. Leaf chlorophyll will be measured using a commercially available chlorophyll meter. Plots will be harvested for biomass production and plant tissue analysis to determine the degree to which inoculation results in improved N use efficiency.