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United States Department of Agriculture

Agricultural Research Service

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Research Project: Developing Rnaseq Diagnostics for Eukaryotic Pathogens of Plants

Location: Foreign Disease-Weed Science

Project Number: 1920-22000-040-09
Project Type: Specific Cooperative Agreement

Start Date: Jun 01, 2014
End Date: May 31, 2015

Objective:
Develop a RNA-based massively parallel sequencing (next generation sequencing) approach for the detection of eukaryotic plant pathogens.

Approach:
1. Develop an effective universal RNA extraction approach for plants. 2. Apply next generation sequencing techniques to the RNA extracted from pathogen infected plants. 3. Adapt existing bioinformatic tools to utilize next generation RNAseq data for diagnosis of eukaryotic plant pathogens.

Last Modified: 9/2/2014
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