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United States Department of Agriculture

Agricultural Research Service

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Research Project: Antimicrobial Activity of Sophorolipid Biosurfactants Against Bacteria Isolated from Salt-Packed Hides and Skins

Location: Biobased and Other Animal Co-Products

Project Number: 1935-41000-090-03
Project Type: Nonfunded Cooperative Agreement

Start Date: Jan 01, 2014
End Date: Sep 30, 2014

Objective:
To test and develop new application of antimicrobial biosurfactants for killing salt-tolerant bacterial that damage hides and skins.

Approach:
Antimicrobial sophorolipid (SL) biosurfactants will be produced by fermentation using yeast strains such as Candida bombicola and Rhodotorula bogoriensis. Structurally different SLs will be obtained by feeding C. bombicola with different substrates such as oleic acid, stearic acid, and palmatic acid. R. bogoriensis, on the other hand, produces SL containing long-chain (22 carbons)SL. The SLs will be used in antimicrobial assay against bacteria isolated in collaborator's lab that are related to degraded hides and skins, such as proteolytic Bacillus mycoides, Aerococcus viridians, and Staphylococcus equorum; lipolytic S. xylosus and Enterobacter sakazakii; proteolytic Eb. cloacae, Pseudomonas luteola, and Vibrio fluvialis; and proteolytic Enterococcus faecium, B. pumilus, B. licheniformis, and S. cohnii. Antimicrobial activities of SLs will be examined on the mix culture of these microorganisms in liquid test media containing 25% NaCl (i.e., brine curing and pre-soak liquor), 3% NaCl (i.e., main soak liquor), and organic substances.

Last Modified: 8/19/2014
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