Project Number: 2050-21000-032-00
Start Date: Mar 01, 2013
End Date: Feb 28, 2018
As this project’s research is non-hypothesis driven, no hypotheses are presented in the approach. Experimental approaches to be used include hybridization of breeding clones of species and their enhanced progeny from ARS collaborators with adapted parental material from our program using a modified backcross method to minimize inbreeding depression. Progenies synthesized will be screened for trait(s) of interest for genetic enhancement, as well as for acceptable agronomic characteristics to identify superior breeding clones having the desired traits of the wild species with better adaptation and agronomic characteristics. Seed amounts are adequate by the end of the 2nd field season to allow field evaluation of promising progenies in a randomized completed block design (RCB) relative to industry standards, with the number of replicates and locations of trials increasing in subsequent field years as a breeding clone advances in the project. Data analysis over years and locations consists of a mixed model ANOVA with repeated measures with fixed effects being clone, year, and their interaction. Random effects would be location, clone x location, block within location, and clone x block within location. The latter interaction would be the experimental unit for which the correlation structure across years would be incorporated in the model. Breeding clones with enhanced traits will be released as new varieties, or alternatively as germplasm for breeding if industry requirements for a new variety are not fully met. Marker assisted selection also will be utilized to accelerate breeding for resistances to viruses and nematodes that impact the western United States. Newly-released potato varieties and advanced breeding lines will also be assayed to determine susceptibility to potato tuber necrotic ringspot disease caused by Potato virus Y (PVY) strains. PVY strain isolates of the O, NTN, and N:O groups will be used to characterize foliar and tuber symptoms in North American cultivars and advanced breeding lines in U.S. potato breeding programs. These experiments will be conducted in a greenhouse secured to prevent aphid entry to eliminate cross-contamination from outside sources and the release of the PVY strains used in the research. Breeding efforts include the “stacking” or “pyramiding” of an assemblage of desirable traits into a variety that proves acceptable to the potato industry. However, if the trait in question proves recalcitrant for effectively combining with other desired traits into an improved potato variety, then the following approaches would be taken: 1. The enhanced breeding clone would be released by the project as germplasm for use as parental material by other potato breeding programs. 2. Continued hybridization by this project of the enhanced breeding clone with potato varieties to obtain commercially-acceptable progeny having the desired multiple genetic enhancements. The number of progenies evaluated from these hybridizations would also be increased to improve the probability of success in identifying superior selections having the desired multiple traits.