Project Number: 5402-21000-007-21
Start Date: May 01, 2012
End Date: Apr 30, 2013
The potato cultivars are deposited at the USDA-ARS, National Center for Genetic Resources Preservation as tissue culture and are maintained in vitro as shoots and tubers as well as in long-term liquid nitrogen tanks as shoot tips. The cryopreservation procedure has evolved over years. Currently, the droplet vitrification method developed by Haeng-Hoon et al. (2006) is the widest protocol used. According to the lab standards, a minimum of 40% viability is required for an accession to be considered successfully cryopreserved at the Center. Procedural modifications are made for genotypes not responding to the established protocols.