Start Date: Nov 11, 2009
End Date: Oct 31, 2013
Apple fruit will be treated and stored under conditioned known to provoke or reduce scald. Broad untargeted metabolic profiling techniques will be used to track symptom develop from disorder inception, through the asymptomatic development stages, and finally, during symptom development in fruit that are expected to, or not expected to, develop symptoms. Metabolomic data will be compiled and modeled using multivariate data-mining techniques to reveal associations among metabolites from multiple pathways and symptom development. Further experimentation will employ further treatment and storage-based contrasts that are expected to link other metabolites to disorder development while validating already discovered prospective metabolic biomarkers.