Start Date: Jul 01, 2009
End Date: Sep 30, 2012
Alates from light traps and workers from inground stations, infestations of trees and structures that are active during the respective swarm season will be collected throughout the French Quarter of New Orleans with the assistance of USDA/ARS and New Orleans Mosquito and Termite Board (N.O.M.& T.C.B). The alates will be counted, sexed and stored and each colony will be identified by its genotypic profile. This allows us to test whether the alates originate from colonies in the vicinity of their capture and establish how often the same colonies swarm during a swarm season and over the years, and how far alates from a particular colony fly. Microsatellite development: DNA will be extracted and digested with restriction enzymes from at least 20 termites per colony from at least 20 colonies per population and multiple populations per geographic region. Protocols for microsatellite genotyping will be optimized for each species. Samples will be assigned to colonies and the breeding system (simple or extended family) and degree of inbreeding will be determined for each colony. Population genetic structure will be described using F-statistics. Genetic distances among colonies and populations will be used to track origin and spread of invasive termites.