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United States Department of Agriculture

Agricultural Research Service

Research Project: TOWARDS DETECTING PROTEIN-PROTEIN INTERACTIONS IN SOYBEAN AND OTHER PLANTS VIA MASS SPECTROMETRY

Location: Soybean Genomics and Improvement

Project Number: 8042-21220-229-04
Project Type: Specific Cooperative Agreement

Start Date: Jul 21, 2008
End Date: Jul 20, 2013

Objective:
Develop methods for targeting and investigating interactions between soybean pathogen proteins and plant proteins involved in disease resistance or other important signaling pathways necessary for optimal plant productivity.

Approach:
Genes from plants and pathogens will be modified to encode a recognizable protein sequence tag. The modified gene will be expressed in plants. The expressed tagged-protein from the transgene will be purified based on tag recognition methodology and approaches will be designed to isolate the tagged protein and it’s potential interacting protein partners. These protein complexes or aggregates will be analyzed by mass spectrometry, which will resolve the individual protein components and thus reveal which proteins are interacting with the tagged protein.

Last Modified: 11/27/2014
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