Location: Foreign Animal Disease Research
Project Number: 1940-32000-057-49
Start Date: Feb 01, 2013
End Date: Jul 31, 2014
1. A comparison study of intranasal instillation and aerosol infection using a commercially available aerosol delivery system to determine the most reproducible way of infecting Merino sheep will be conducted. 2. A delivery study utilizing decreasing doses of emergency serotype O vaccine via the intra-muscular and intra-dermal routes will be conducted. The immune responses of each will be studied using in vitro assays. 3. In vivo vaccination studies will be conducted using the best methods determined above and challenge with a heterlogous virus to determine vaccine efficacy. 4. To determine carrier state activity, vaccinated and infected sheep will be studied for at least 35 days post infection. Studies will also include minimal protective dose.