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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic and Transcriptomic Analysis of Postharvest Decay Resistance in M. Sieversii and Identification of Pathogenicity Effectors in P. Expansum

Location: Appalachian Fruit Research Laboratory: Innovative Fruit Production, Improvement and Protection

Project Number: 1931-21000-022-04
Project Type: Reimbursable

Start Date: Mar 01, 2013
End Date: Dec 01, 2013

Objective:
The objectives of this project are: 1) identify potential effector genes in P. expansum that play a role in pathogenicity; and 2) identify QTLs for disease resistance in apple using a segrating mapping population of Malus sieversii x 'Royal Gala' (GMAL4593).

Approach:
Comparative secretome analysis and BLAST sequence similarity searches will be used to identify putative effectors in the sequenced genome of P. expansum. Transcriptomic analysis using Illumina technology will be used to examine differential gene expression in resistant and susceptible apple genotypes of M. sieversii in response to P. expansum. QTL analysis will be performed independently and combined for both parents in the GMAL4593 population to identify loci affecting P. expansum. Marker density in the QTLs will be increased using additional SNPs and DIPs. Identification of candidate resistance genes located in the QTL will be done by examining the sequenced genome of 'Golden Delicious'.

Last Modified: 8/19/2014
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