1a.Objectives (from AD-416):
The objective is to identify the regions of the soybean genome that have been changed or retained through 30 years of selection for yield in a set of 140 high yielding soybean lines that have been developed using germplasm accessions that are distinct from the set of accessions that form the genetic base of currently grown U.S. soybean varieties.
1b.Approach (from AD-416):
The 50,000 Illumina iSelect Beadchip developed at the USDA, Beltsville will be used to characterize 140 high yielding experimental lines that produce yields equal to or superior to currently grown U.S. soybean varieties. Using these data from the 50,000 Illumina iSelect SNPs we will determine the genomic changes that have occurred by tracking the regions of the genome that have been changed or retained through 3 decades of selection via a comparison with the 54 exotic accessions that were used as parents and that have already been genetically analyzed as part of an ongoing United Soybean Board funded project that has used the 50,000 SNP Illumina Beadchip to characterize the entire USDA Soybean Germplasm Collection.
Funds are provided by the United Soybean Board, as part of Project #2219 entitled “Characterizing an alternative gene pool for increasing U.S. soybean yield”. This project is being undertaken for the purpose of determining genetic factors that have changed as a result of 30 years of breeding for high productivity using “exotic” genetic materials from the USDA Soybean Germplasm Collection. A total of 140 high yielding experimental lines derived from this breeding program will be analyzed with the custom Illumina beadchip that contains more than 50,000 single nucleotide polymorphism (SNP) DNA markers. These lines will all have been demonstrated to produce seed yields equal to the best U.S. soybean varieties. The DNA marker data from the 140 high yielding soybean lines developed from exotic genetic materials will be compared with similar DNA marker data of high yielding commercial varieties. The goal is to identify those regions of the soybean genome that are positively impacting yield. Progress was made in the preparation of DNA from the 140 high yielding soybean lines that were derived from exotic soybean germplasm. This DNA is currently being prepared for analysis with the 50,000 SNP DNA markers on the custom Illumina beadchip. The resulting data will be compared with previously obtained SNP DNA marker data on high yielding commercial varieties.