Comparative evaluation of new resistance sources and development of field-based serological detection of CYSDV
Crop Improvement and Protection Research
Project Number: 5305-22000-013-03
General Cooperative Agreement
Start Date: Jun 15, 2012
End Date: Dec 31, 2012
Evaluate exotic melon germplasm from India for potential new sources of resistance to CYSDV; Characterize host plant resistance to CYSDV in PI 313970 and TGR-1551, and select and introgress resistance to western U.S. shipping type background adapted to the desert southwest U.S.; Evaluate virus content in PI 313970, TGR-1551 and lines derived from these sources to determine ability of lines to suppress CYSDV accumulation; and develop antiserum for field diagnosis of CYSDV using an immunostrip format.
Resistant, susceptible and segregating germplasm will be field tested for resistance to CYSDV in field trials and tested for virus by molecular method. Resistant and susceptible germplasm will be grown under controlled conditions, inoculated in standardized tests and screened for virus concentration and transmission efficiency by whiteflies. Virus protein will be expressed and antiserum developed for use in immunodetection of CYSDV.