1a.Objectives (from AD-416):
Provide identifications of nematodes submitted by APHIS personnel, as well as related expertise.
1b.Approach (from AD-416):
Perform microscopic and molecular identifications of intercepted nematodes. Provide information about geographic distribution and host range.
Because intercepting dangerous foreign organisms in imported agricultural and related materials is critical to the success of American agriculture, the work performed in this subordinate project serves to provide nematode identifications and related expertise as requested by the USDA Animal and Plant Health Inspection Service. In turn, the identification of these nematodes contributes to achievement of an objective of the parent project, specifically to discover new nematode species, new host associations, and new geographic occurrences. During FY 2013 accurate nematode identifications and related expertise were provided for 559 plant and soil samples (493 of them urgent) intercepted by APHIS at ports-of-entry or during domestic surveys. Domestic surveys included national surveys for the presence of potato pale cyst nematodes and surveys in New York to delineate the distribution of golden nematode and the viability of cysts following soil fumigation. Nematodes identified from plant materials intercepted at ports of entry included a variety of cyst, root-knot, lesion, dagger, ring, foliar, chrysanthemum, stubby-root, burrowing, stunt, and spiral nematodes. Related expertise provided to APHIS included information about the distribution and pathogenicity of many of these nematodes as well as potentially invasive nematode species, information on morphological and molecular protocols useful for identification, and information about which nematode species pose threats to agriculture. The results of these identifications were used by APHIS for taking appropriate regulatory actions beneficial to growers and the public.