1a.Objectives (from AD-416):
To determine how salmon consumption alters lipid oxidation parameters in human plasma. In this work, we will quantify the content of omega-6 and omega-3 fatty acids in the plasma from participants in the GFHNRC human study that have consumed differing amounts of salmon as a source of omega-3 fatty acids. These data will provide information on the role of fish consumption upon markers of inflammation and oxidative stress. Attainment of this data may be useful in future deliberations of the dietary guidelines committee on the evidence base for long chain omega-3 fatty acid recommended intake levels.
1b.Approach (from AD-416):
The GFHNRC is responsible for performance of studies to assess the role of nutrition in obesity prevention and in the reduction of obesity related chronic diseases. The GFHNRC will perform the necessary salmon feeding trial and collection of plasma samples. Samples of plasma will be delivered to the cooperator for analysis of fatty acid oxidation products. The cooperator will also offer expertise in interpretation and writing of the results. Data will be presented and published with GFHNRC scientists and the cooperator as authors. The cooperator will also offer expertise in interpretation of the results of the phospholipid fatty acids.
The intervention has been completed and most of the samples have been analyzed. The study has thus far resulted in two papers – one that is in press and one that is currently under review. The samples of plasma for analysis of fatty acid oxidation products have been delivered to the cooperator and are currently being analyzed. Once these results are available, a manuscript of the results well be prepared for submission.